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ASSESSMENT OF Fe2O3 NANOPARTICLES IMPACT ON FUNCTIONAL ACTIVITY OF RATS’ PERITONEAL MACROPHAGES IN EXPERIMENTS IN VITRO AND IN VIVO

ASSESSMENT OF Fe2O3 NANOPARTICLES IMPACT ON FUNCTIONAL ACTIVITY OF RATS’ PERITONEAL MACROPHAGES IN EXPERIMENTS IN VITRO AND IN VIVO

https://doi.org/10.33573/ujoh2015.03.028

Dmytrukha N. M., Lugovskoy S. P., Lahutina O. S.

ASSESSMENT OF Fe2O3 NANOPARTICLES IMPACT ON FUNCTIONAL ACTIVITY OF RATS’ PERITONEAL MACROPHAGES IN EXPERIMENTS IN VITRO AND IN VIVO

SI "Institute for Occupational Health of National Academy of Medical Sciences of Ukraine", Kyiv

Full article (PDF), ENG

Introduction. Today much attention is paid in the world to studying safety of nanomaterials (NM), which are synthesized and used in different spheres of human activity. Among metallic nanoparticles (NPs), iron and iron oxides are widely used in medicine and pharmacy. An important step in learning, how NPs affect living organisms, is studying their toxicity and biological activity. Macrophages are of a particular interest in this respect, which are available in various tissues and which are key cells in forming an immune response.

Purpose of the study. To assess Fe2O3 NPs influence on viability and functional activity of peritoneal macrophages in rats in experiments in vitro and in vivo.

Materials and methods. Fe2O3 NPs of 19 nm and 75 nm were obtained by chemical methods. The viability of peritoneal macrophages under the influence of Fe2O3 NPs of 19 nm and 75 nm was defined by trypan blue and MTT test; the phagocytic activity was assessed by absorption of latex particles and cytochemically; bactericidity – by NBT-test.

Results. The studies showed that incubation of macrophages with NPs in vitro caused death of 30 % cells, whereas entering NPs into the body in vivo had no effect on their viability. NPs activated macrophage phagocytosis, formation of large phagocytic vacuoles, fagosom, lysosomes and fagolizosom as well as production of reactive oxygen forms. NPs activity depends on their size and conditions of experiments.

Conclusion. The contact of Fe2O3 NPs with macrophages activates phagocytosis and formation of reactive oxygen forms, which are aimed at their elimination and neutralization. However, excessive or chronic stimulation of an oxidative stress can lead to cell death, the inflammatory process. Stimulation of the biological activity of macrophages by Fe2O3 NPs makes it possible to suppose their possible toxic effect on the immune system and requires further immunological studies.

Key words: nanoparticles of iron oxide, peritoneal macrophages, рhagocytosis

References

  1. Yokel, R. A., Macphail, R. C. 2011. “Engineered nanomaterials: exposures, hazards, and risk prevention”, J. Occup Med Toxicol., no. 6, pp. 7–13. https://doi.org/10.1186/1745-6673-6-7
  2. Buzea, C., Blandino, I. I. P., Robbie, K. 2001. “Nanomaterials and nanoparticles: sources and toxicity”, Biointerphases, v. 2, no. 4, pp. 17–71. https://doi.org/10.1116/1.2815690
  3. Faraji, M., Yamini, Y., Rezaee, M. 2010. “Magnetic nanoparticles: synthesis, stabilization, functionalization, characterization, and applications”, J. Iran. Chem. Soc., v. 7, no. 1, pp. 1–37.
  4. Katsnelson, B. A., Privalova, L. I., Sutunkova, M. P. et al. 2012,“Uptake of some metallic nanoparticles by and their impact on pulmonary macrophages in vivo as viewed by optical, atomic force and transmission electron microscopy”, J. Nanomed. & Nanotech., v. 3, no. 1, pp. 1–9.
  5. Dobrovolskaia, M. 2007, “Immunological properties of engineered nanomaterials”, Nat. Nanotechnol., no. 2, pp. 469–478. https://doi.org/10.1038/nnano.2007.223
  6. Park, J. B. Phagocytosis induced superoxide formation and apoptosis in macrophages, 2003, Exp. Mol. Med., v. 35, no. 5, pp. 325–335. https://doi.org/10.1038/emm.2003.44
  7. Carlson, C., Hussain, S. M., Schrand, A. M. [et al.]. 2008, “Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species”, J. Phys. Chem. B., v. 112, no. 43, pp. 13608–13619. https://doi.org/10.1021/jp712087m
  8. Park, J., Lim, D. H., Lim, H. J. [et al.]. 2011, “Size dependent macrophage responses and toxicological effects of Ag nanoparticles”, Chem.Commun. (Camb), no. 47, pp. 4382–4384. https://doi.org/10.1039/c1cc10357a
  9. Metz, S., Bonaterra, G., Rudelius, M. [et al.]. 2004, “Capacity of human monocytes to phagocytose approved iron oxide MR contrast agents in vitro”, Eur. Radio., v. 14, no. 10, pp. 1851–1858. https://doi.org/10.1007/s00330-004-2405-2
  10. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. 1986. Strasbourg, http:// conventions.coe.int/treaty/en/treaties/html/123.htm
  11. Basic cell culture protocols. Third edition. 2005. Ed. by Cheryl D. Helgason, Cindy L. Miller / Human Press Inc., Totowa New Jersey, v. 290, 371 p.
  12. Freeman R., King B., 1972. "Technique for the performance of the nitro-blue tetrazolium (NBT) test", J. of Clinical Pathology, v. 25, no. 10, pp. 912–914. https://doi.org/10.1136/jcp.25.10.912
  13. Lojda, Z., Gossrau, R., Schiebler, T.H. 1982, “En zym e Histochemistry. A Laboratory manual”, Мoskva : Mir, 259 p.
  14. De Brauwer, E., Jacobs, J., Nieman, F. [et al.]. 1999,“Test Characteristics of Acridine Orange, Gram, and May-Grünwald-Giemsa Stains for Enumeration of Intracellular Organisms in Bronchoalveolar Lavage Fluid”, J. Сlinical microbiology, v. 37, no. 2, рр. 427–429.
  15. Sioutas, Li. N., Cho, C., Schmitz, A. [et al.]. 2003, ”Ultrafine particulate polutants induced oxidative stress and mitochondrial damage”, Environ. Health Perspect., v. 111, no. 4, рр. 455–460. https://doi.org/10.1289/ehp.6000
  16. Brown, D. M., Donaldson, K., Borm, P. J. [et al.]. 2004, ”Calcium and ROS-mediated activation of transcription factors and TNF-alpha cytokine gene exptession in macrophages exposed to ultrafine particles”, Am. J. Physiol. Lung Cell Mol. Physiol., v. 286, no. 24, рр. 344–353. https://doi.org/10.1152/ajplung.00139.2003