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DISORDERS OF IRON METABOLISM IN LEAD EXPOSURE (CLINICAL AND EXPERIMENTAL STUDIES)

DISORDERS OF IRON METABOLISM IN LEAD EXPOSURE (CLINICAL AND EXPERIMENTAL STUDIES)

https://doi.org/10.33573/ujoh2016.01.031

Lubyanova I. P. 1, Lugovskiy S. P. 1, Mykhaylyk O. M. 2, Dudchenko N. A.3

DISORDERS OF IRON METABOLISM IN LEAD EXPOSURE (CLINICAL AND EXPERIMENTAL STUDIES)

1State Institution "Institute for Occupational Health of the National Academy of Medical Sciences of Ukraine", Kyiv

2Ethris GmbH, Planegg, Germany

3M. P. Semenenko Institute of Geochemistry, Mineralogy and Ore Formation of the National Academy of Sciences of Ukraine, Kyiv

Full article (PDF), ENG

Actuality. A problem of the part of lead in development of body iron overload is actively discussed in the modern literature. In this, disorders of heme and nonheme iron in the body in lead intoxications is still not sufficiently investigated.

Purpose of the work. To study peculiarities of disorders of heme and nonheme iron in workers, exposed to lead in production conditions as well as to investigate morphological manifestations of hemosiderosis on an experimental model of lead intoxication in rats.

Materials and methods. 19 workers of an art glass factory, aged 42 ± 11 (main group), occupationally exposed to lead as well as 17 workers of the control group, aged 40 ± 14, were subjects on the study. Indices of iron metabolism (transferrin iron concentration [Tf-Fe], transferrin protein concentration [Tf], transferrin saturation [% Tf], ferritin iron [Ft-Fe] and chelatable iron [Df-Fe] concentration in blood, transferrin iron concentration in blood cells, transferrin and ferritin iron concentrations in plasma, iron concentration in serum) were studied, using a quantitative method of electronic spin resonance (ESR). Also, 36 Vistar male rats were used for reproducing a model of acute, subacute and chronic lead intoxication for morphological assessment of iron (III) metabolism disorders. For this purpose, histological slides were prepared from the liver pieces, filled into paraffin, with the use of microtome and Pearl’ reaction.

Results. It is seen that occupational contact with lead results in a significant increase of serum iron concentration, transferrin iron concentration in blood cells and transferrin saturation, promoting development of a body iron overload. The results of experiments is the evidence of this, where development of expressiveness of interstitial hemosiderosis of various degree has been stated.

Key words: lead poisoning, experimental lead intoxication, transferrine, ferritin, hemosiderosis, electronic spin resonance, secondary iron overload

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