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MORPHOLOGICAL CHARACTERISTICS OF HEPATOTOXIC EFFECTS OF STABLE STRONTIUM CHLORIDE IN CHRONIC EXPERIMENTS IN RATS

https://doi.org/10.33573/ujoh2015.02.026

Lugоvskyi S. P.1, [Shlopov V. G.] 2, Sudya D. A.3

MORPHOLOGICAL CHARACTERISTICS OF HEPATOTOXIC EFFECTS OF STABLE STRONTIUM CHLORIDE IN CHRONIC EXPERIMENTS IN RATS

1SI "Institute for Occupational Health of NAMS of Ukraine", Kiev

2Donetsk National Medical University

3Odessa National Medical University

Full article (PDF), ENG

Background. In recent years, stable strontium and its compounds have found their wide application in various branches such as industry, agriculture, medicine, as well as in everyday life. However, aspects of the toxic effects of metal and its compounds on the body are not yet fully understood.

Purpose of the study. To study hepatotoxicity effects of stable strontium chloride in chronic experiments in rats, according to morpho-functional data.

Materials and methods. An experimental model of chronic strontium intoxication was used on male rats (n = 36), daily (5 days a week for 4 months) in intragastric gavages of an aqueous solution of strontium chloride in the stable dose of 1/30 DL50. The hepatotoxic evaluation of stable strontium chloride effects was made according to histological and histochemical study on rat liver after 1, 2, 3 and 4 months. For this purpose histological specimens were stained with hematoxylin & eosin, toluidine blue, ammoniac solution of silver nitrate, mixture of sudan III & IV. Histochemical reactions were carried out: PAS (period ic acid–Schiff) in combination with diastase (PAS-D stain) for detection of glycogen, as well as the coupling with naphthol AS- BS- phosphate for detecting the activity of acid phosphatase (AP).

Results. The studies have shown that chronic exposure to low doses of stable strontium chloride, are accompanied by development of hepatotoxic effects, which are characterized by: blood circulation impairment (blood stasis in hepatic veins and sinusoidal capillaries, especially in hepatic acini zone III); stromal edema and organ hyalinosis with collagen fibers; lipo- and protein dystrophy of hepatocytes, developed due to the reduced amount of glycogen in hepatocytes; hypertrophy and hyperplasia of Kupffer cells, as well as the increase in the amount of Kupffer cells in hepatocytes, due to histochemical activity of AP.

Conclusion. Chronic exposures to stable strontium chloride in small doses, at 1/30 DL50 causes development of drastic effects due to hepatotoxic metal action, which should be taken into consideration ,when assessing the risk of exposure to this metal both for industrial and household environment.

Key words: stable strontium, intoxication, experiment, hepatotoxic effects

References

  1. http://www.inchem.org/documents/cicads/cicads/cicad77.pdf., 2010, IPCS. Strontium and strontium compounds: Concise International Chemical Assessment Document 77. WHO, Geneva.
  2. Nokhrina, O. I., Rozhihina, I. D., Dmitrieynko, V. I., Platonov, M. A. 2012, "Doping and modifying steel with natural materials", Tomsky Polytechnical University: Tomsk, 320 p. (in Russian).
  3. Petrenko, D. V. 2014, "Effect of phosphate fertilizer on the content of strontium in landscapes" (Dissertation). Moscow, 159 p. (in Russian).
  4. Reginster, J. Y. 2002, "Strontium ranelate in osteoporosis", Curr. Pharm. Des., no. 8, pp. 1907–1916.https://doi.org/10.2174/1381612023393639
  5. Sudya, D. A. 2013, "Problems of toxic influence of stable strontium salts on the body (review)", Modern problems of toxicology, food and chemical safety, no. 3, pp. 55–60 (in Russian).
  6. European Convention for the protection of vertebrate animals, used for experimental and other scientific purposes, 1986, Strasbourg: Council of Europe, 53 p.
  7. Microscopic technique (Manual). 1996, (Ed. D. S. Sar­kisov and Yu. Perov). Moscow: Meditsina, 544 p. (in Russian).
  8. Lloyda, H., Gossrau, R., Shibler, T. 1982, "Enzymes histochemistry. Laboratory methods" (translated from English by I.B. Bukhvalova and others), Ed. N. T. Raichlin. Moscow : Mir, 270 p. (in Russian).
  9. Gubler, E. V. 1978, Computational methods of analysis and detection of pathological processes. Leningrad : Meditsina, 296 p. (in Russian).
  10. Ryazantseva, N. V., Novitsky, V. V., Stepovaya, E. A., Tkachenko, S. B. 2004, "Erythrocytes in pathology: Reflections from an electron microscope", Archives of Pathology, no. 3, pp. 53–61 (in Russian).
  11. Aruin, L. I., Babayev, B., Gelfand, V. B. et al. 1987, Basic structure of adaptation and compensation of disturbed functions (Eds. D.S. Sarkisov). Moscow : Meditsina, 448 p. (in Russian).
  12. Zyuzyukin, Y. 1976, "To the problem on toxic properties of strontium in inhalations", Gigiyena i sanitaria, no. 8, pp. 99–100 ( in Russian).
  13. Shafran, L. M., Large, D. V., Pykhteyeva, E. G., et al. 2004, "The role of lysosomes in the mechanism of protection and cell damage under the influence of heavy metals", Modern problems of toxicology, no. 3, pp. 17–24 (in Russian).