Dmytrukha N. M., Lugovskoy S. P., Lahutina O. S.
ASSESSMENT OF Fe2O3 NANOPARTICLES IMPACT ON FUNCTIONAL ACTIVITY OF RATS’ PERITONEAL MACROPHAGES IN EXPERIMENTS IN VITRO AND IN VIVO
SI "Institute for Occupational Health of National Academy of Medical Sciences of Ukraine", Kyiv
Full article (PDF), ENG
Introduction. Today much attention is paid in the world to studying safety of nanomaterials (NM), which are synthesized and used in different spheres of human activity. Among metallic nanoparticles (NPs), iron and iron oxides are widely used in medicine and pharmacy. An important step in learning, how NPs affect living organisms, is studying their toxicity and biological activity. Macrophages are of a particular interest in this respect, which are available in various tissues and which are key cells in forming an immune response.
Purpose of the study. To assess Fe2O3 NPs influence on viability and functional activity of peritoneal macrophages in rats in experiments in vitro and in vivo.
Materials and methods. Fe2O3 NPs of 19 nm and 75 nm were obtained by chemical methods. The viability of peritoneal macrophages under the influence of Fe2O3 NPs of 19 nm and 75 nm was defined by trypan blue and MTT test; the phagocytic activity was assessed by absorption of latex particles and cytochemically; bactericidity – by NBT-test.
Results. The studies showed that incubation of macrophages with NPs in vitro caused death of 30 % cells, whereas entering NPs into the body in vivo had no effect on their viability. NPs activated macrophage phagocytosis, formation of large phagocytic vacuoles, fagosom, lysosomes and fagolizosom as well as production of reactive oxygen forms. NPs activity depends on their size and conditions of experiments.
Conclusion. The contact of Fe2O3 NPs with macrophages activates phagocytosis and formation of reactive oxygen forms, which are aimed at their elimination and neutralization. However, excessive or chronic stimulation of an oxidative stress can lead to cell death, the inflammatory process. Stimulation of the biological activity of macrophages by Fe2O3 NPs makes it possible to suppose their possible toxic effect on the immune system and requires further immunological studies.
Key words: nanoparticles of iron oxide, peritoneal macrophages, рhagocytosis
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