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ASSOCIATION BETWEEN CSTTI, GSTM1, СС1Б, TNF-a, Pi GENETIC POLYMORPHISMS AND COAL WORKERS' PNEUMOCONIOSIS

DOI xxxxxxxx

Gorovenko N.G.1, Zhurakhovskaya N.V.1*2, Basanets A.V.2, Podolskaya S.V.1

ASSOCIATION BETWEEN CSTTI, GSTM1, СС1Б, TNF-a, Pi GENETIC POLYMORPHISMS AND COAL WORKERS' PNEUMOCONIOSIS

1National Medical Academy of Post-Graduate Education named after P.L Shupyk, Kyiv

2Institute for Occupational Health of AMS of Ukraine, Kyiv

Full article (PDF), UKR

Coal Workers Pneumoconiosis (CWP) is a multifactorial disease arising as a result of the combined action of genetic and exogenous factors. Risk estimation of the origin of this disease by the detection of group of persons with the inherited sus-ceptibility to CWP is important for prophylaxis of CWP development. Basing on mechanisms of CWP pathogenesis the most real candidates for the role of biomarkers of the inherited susceptibility to this disease are genes GST, TNF-a, CC16 and Pi. The polymorphic alleles of the genes GSTT1, GSTM1, TNF-a, CC16 and Pi were investigated by a molecular-genetic method in 112 miners with CWP and 138 healthy miners (control group), that have minimum 13 years of the underground work experience. The GSTM1 and GSTT1 alleles were analyzed by a multiplex PCR method. The polymorphism of TNF-a, CC16 and Pi genes was identified by restriction fragment-length polymorphism (RFLP). The statistical analysis of results was executed by the use of the y} and Z-criterion methods.The difference between frequency of the Pi*MM and Pi*MZ genotypes in the controls and in the CWP patients group was not statistically significant (P = 0,19). A ZZ homozygous variants were not found in the examined groups. The analysis of genes alleles combinations GSTT1, GSTM1, CC16, Pi and TNF-a shows that the frequency of distribution genotypes GSTM1(-/-);CC16(A38G)*A/A (Z=2,13, P = 0,034) and GSTTl(-/-);GSTMl(-/-);TNF-a-308*G/G (Z=l,99, P = 0,046) was statistically significantly greater in the control group. A frequency of genotype GSTTl(-/-);GSTMl(+);TNF-a- 308*G/A (Z=2,96; P = 0,003) was considerably higher in the CWP patients group.

Key words: pneumoconiosis, polymorphism, genes GSTT1, GSTM1, TNF-a, CC16, Pi, Z-test method

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