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Changes in expression of melatonin MT1 receptors in the general cover of rats caused by dark deprivation

Changes in expression of melatonin MT1 receptors in the general cover of rats caused by dark deprivation

ISSN 2223-6775 Ukrainian journal of occupational health Vol.16, No 2, 2020

Changes in expression of melatonin MT1 receptors in the general cover of rats caused by dark deprivation

Sobolevskaya I.S., Krasnobaeva M.I., Myadelets O.D.

https://doi.org/10.33573/ujoh2020.02.143

Vitebsk State Medical University, Belarus

Full article (PDF), UKR

Introduction. All major types of skin cells have an autonomous functional circadian system that reflects certain periods and phase relationships in the expression of genes and proteins. A special role in the functioning of the general cover is also played by the “clock” hormone of the pineal gland melatonin, which acts on target cells through melatonin receptors.

The aim of the study was to study the dynamics of the expression of melatonin MT1 receptor in white male rats during dark deprivation.

Materials and research methods. In the experiments, 20 white outbred male rats with a body weight of 170–220g at the age of 3 months were used. In accordance with the experimental design, the experimental animals were randomly divided into 2 groups: intact — animals under standard fixed lighting conditions (12 hours light / 12 hours darkness); animals with modeling of dark deprivation in conditions of the round-the-clock lighting (24 hr light). On days 7, 14, and 21, histological material was collected (fragments of the skin of the interscapular region of the back). For immune-histochemical studies, serial sections were stained using MTNR1A polyclonal antibodies. For morphometric data analysis, the Image Scope Color computer program was used, with which the fill factor (%) was automatically estimated. All statistical data processing was performed using the Statistica 10.0 software. Differences were considered significant at the level of less than 0.01 (p <0.01).

Results. Throughout the experiment, the expression of melatonin MT1 receptors in the cells of the epidermis and hair follicles changed in waves: at first a sharp increase in the expression of melatonin receptors was observed (7th day), then there was a slight decrease (14th day), and by the 21st day a sharp increase. Moreover, in the cells of the sebaceous glands, a steady increase in the level of expression throughout the experiment can be observed.

Conclusions. Long-term dark deprivation leads to the increase in the expression of cytoplasmic MT1 receptors in the cells of the epidermis, sebaceous glands and hair follicles; an increase in the number of immunopositive cells is directly proportional to the duration of the experiment.

Keywords: dark deprivation, melatonin receptors, skin, epidermis, sebaceous glands, hair follicles

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