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Chemical-analytical characteristics of different phases of rats striated muscles regeneration under chronic hyperglycemia

ISSN 2223-6775 Ukrainian journal of occupational health Vol.16, No 1, 2020

Chemical-analytical characteristics of different phases of rats striated muscles regeneration under chronic hyperglycemia

Rtail R., Tkach G.

Sumy State University, Sumy, Ukraine

Full article (PDF), УКР

Introduction. Today, mechanical trauma is the main cause of skeletal muscle injury. Along with this, diabetes mellitus is one of the most widespread pathologies, which is especially common in industrialized countries. However, to date, there are practically no data about changes of micro- and macroelements composition of skeletal muscles in their post-traumatic recovery under chronic hyperglycemia (CH) condition.

The purpose of the study was to determine the content of K, Na, Ca, Mg, Fe, Zn, Cu in the post-traumatic skeletal muscle regenerates of rats with CH.

Materials and methods. 80 laboratory white adult male rats (7-9 months) were used in the experiment: 40 animals – a control group; 40 animals – an experimental group (animals with modeled CH). A mechanical injury in both groups was reproduced on triceps shin muscle. Animals were removed from experiment on 3, 7, 14, and 28 days. The content of K, Na, Ca, Mg, Fe, Zn, Cu in skeletal muscle regenerates was determined by atomic absorption spectrometry with electrothermal and flame atomization. A mathematical analysis of the obtained data was made using a package SPSS 17.0.

Results. On the 28th day, the difference in chemical elements concentration in regenerating skeletal muscles was not found only for K (P = 0.176). At the same time, the content of Na and Mg in regenerates of animals with CH was higher by 17.3 % (P = 0.031) and 28.2 % (P = 0.015), respectively, compared to the control group. Ca content in places of restorative myogenesis of rats with CH was lower by 13.2 % (P = 0.008), Fe concentration – by 9.7 % (P = 0.025), Zn content – by 78.8 % (P <0.001), and Cu content – by19.4 % (P = 0.033) than in those of the control animals.

Conclusions. So, the increased amount of Na and Mg was found in places of muscles regeneration of rats with CH. This may be the evidence of more pronounced and prolonged destruction of cellular structures. Also, decreased concentration of Ca, Fe, Zn and Cu was revealed in the areas of skeletal muscles regeneration of rats with CH. The reduction of the amount of the mentioned elements may be considered as the consequence of the impaired blood supply of the place of neohistogenesis and the result of general metabolic disorders.

Keywords: hyperglycemia, macroelements, trace elements, skeletal muscle, regeneration, rats


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