DOI xxxxxxxx

Minchenko D.O., Yavorovsky O.P., Zavgorodny I.V., Paustovsky Y.O., Tsuchihara K., Esumi H., Minchenko O.H.

EXPERIMENTAL DATA ON DISORDERS OF EXPRESSION OF CIRCADIAN GENES IN UVER AND LUNGS AS A MARKER OF METHYL-TRETBUTYL ETHER TOXIC EFFECT ON THE BODY

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The development of physiological and biochemical processes in the body is of a circadian character and disorders in their reg-ulation can result in occurrence of different pathological processes, formation of malignant tumours, in particular. The Per2, BMall, Clock and caseinkinase-le are important regulators, which control these processes in normal and pathological con-ditions. We have shown that methyl-tretbutyl ether (MTBE), a toxic and ecologically dangerous chemical compound, can change significantly the expression of Per2, Clock and caseinkinase-le genes in rat liver and lungs. The disorders of such gene expression can destroy the cellular signal pathways and lead to development of pathological processes. The results of our investigation clearly demonstrate that MTBE cause a significant effect on important regulatory mechanisms, which control cell metabolism via circadian gene expression, Per2, Clock and caseinkinase-le, in particular. The expression of circadian genes can be used as a possible sensitive test for detection of the toxic effect of ecologically dangerous chemical compounds on the body.

Key words: liver, genes, expression, methyl-tretbuthyl ether

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