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Experimental study of the influence of Fe2O3 nanoparticles on the rats isolated and in the background of application of the preparation «Quercetin»

Experimental study of the influence of Fe2O3 nanoparticles on the rats isolated and in the background of application of the preparation «Quercetin»

ISSN 2223-6775 Ukrainian journal of occupational health Vol.17, No 1, 2021

Experimental study of the influence of Fe2O3 nanoparticles on the rats isolated and in the background of application of the preparation «Quercetin»

Trakhtenberg I.M., Dmytrukha N.M., Korolenko T.K., Lahutina O.S. Bakalo L.V., Lehkostup L.A.

https://doi.org/10.33573/ujoh2021.01.003

State Institution "Kundiiev Institute of Occupational Health of the National Academy of Medical Sciences of Ukraine", Kyiv

Full article (PDF)

Introduction. Nanoparticles (NPs) of iron oxides during their production and use can enter the human body with air, food and medicine. Given the high biological activity of LF, an important issue for preventive medicine is the study of their toxicity and justification of means of prevention of adverse effects.

The purpose of the study was to determine the toxicity of Fe2O3 solutions with particles of different sizes and an assessment of the effectiveness of using the drug "Quercetin" as a means of preventing their negative effects on the body.

Materials and methods of research. The experiment was performed on adult male Wistar rats weighing 160–180 g, which were in vivarium conditions on standard food and water regimes. Rats were divided into 6 experimental and 2 control groups. The first 3 experimental groups were administered in isolation colloidal solutions of Fe2O3 with a particle size of 19 nm, 75 nm and 400 nm (iron concentration of 0.112 mg /ml), the other 3 groups were injected with colloidal solutions of Fe2O3 on the background of the drug "Quercetin" (4 mg per 100 g of body weight). Colloidal solutions of Fe2O3 were injected into the peritoneum of rats 5 times a week. Control rats were injected with NPs stabilizer (0.1% gelatin solution) in isolation and on the background of Quercetin use. In rats, body weight was determined in dynamics. After 30 injections of Fe2O3, blood and organs were taken. Hematological analysis was carried out with the parameters of red and white blood; determined biochemical parameters characterizing the state of the liver, kidneys, protein and lipid metabolism; assessed the activity of peritoneal macrophages and the level of circulating immune complexes (CIC) in the blood.

Results. It was found that the isolated injection of all Fe2O3 solutions to rats caused an increase in body weight in the dynamics of the experiment. Changes in hematological parameters were characterized by a decrease in hemoglobin, erythrocytes, lymphocytes and granulocytes numbers, an increase of zinc protoporphyrin level and monocytes, bactericidal activity of macrophages and the level of CIC. The data obtained indicate the development of anemia and activation of cells of nonspecific natural immunity. Colloidal solutions of Fe2O3 had a toxic effect on liver and kidney cells, more pronounced after the introduction of NPs Fe2O3 19 nm. The drug "Quercetin" simultaneously with the injection of Fe2O3 solutions contributed to the normalization of certain hematological and biochemical parameters, which indicates a reduction of its toxic effects on peripheral blood, liver and kidneys. The most pronounced prophylactic effect from the use of Quercetin was found in a group of rats injected with NPs Fe2O3 19 nm.

Conclusions. Violation of the cellular composition of the blood, increased activity of intracellular enzymes, the formation of CIC are signs of toxic lesions of organs and tissues, indicating the potential hemato-, hepato- and nephrotoxic effects of Fe2O3 solutions, especially NPs 19 nm. The drug "Quercetin", which was used by rats at the same time with the injection of Fe2O3 colloidal solutions had a membrane-protective, antioxidant and detoxifying effect, which allows us to recommend it for the prevention of the negative effects of NPs Fe2O3 on the body.

Key words: Fe2O3, nanoparticles, toxicity, Quercetin, prevention.

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