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MICRO- AND MACROELEMENTS CONTENT IN RATS’ LIVER AND KIDNEYS AFTER EXPOSURE TO CADMIUM SULFIDE NANOPARTICLES AND CADMIUM CHLORIDE

https://doi.org/10.33573/ujoh2017.03.060

Apykhtina O. L., Kozlov K. P.

MICRO- AND MACROELEMENTS CONTENT IN RATS’ LIVER AND KIDNEYS AFTER EXPOSURE TO CADMIUM SULFIDE NANOPARTICLES AND CADMIUM CHLORIDE

State Institution "Institute for Occupational Health of the National Academy of Medical Sciences of Ukraine", Kyiv

Full article (PDF), ENG

Introduction. The introduction of cadmium nanoparticles (NP) in production makes it necessary to study the mechanism of their action both at the cellular level and at the level of organs and systems, to determine biomarkers of their effects, especially as comparing to their ionic forms.

The aim of the study was – to compare the content of micro and macro elements in the liver and kidneys of experimental animals after exposure to cadmium sulfide NP and cadmium chloride.

Materials and methods. The study was conducted on mature male Wistar rats 160–180 g, intraperitoneally injected with CdS NP of 4–6 nm of 9–11 nm size as well as CdCl2 in 0,08 mg/kg/day dose adjusted to cadmium. Toxic effects were evaluated after 30 injections (1,5 months), 60 injections (3,0 months) and in the post-exposure period (1,5 months after cessation of the exposure).

Results. In the liver of experimental animals the most intensive accumulation of cadmium was observed after exposure to CdCl2, especially in the post-exposure period. There was a higher content of cadmium after exposure to CdS NP–6 nm, as compare to CdS NP 9–11 nm. Along with the increase in the cadmium content in the liver, the growth of the content of copper, iron (more intensively with CdCl2), iron, and lower selenium levels were recorded (especially for CdS NP 4–6 nm). Significant accumulation of cadmium was recorded in the kidneys, especially in the post-exposure period, which can indicate an intensive elimination from the body. A significant accumulation of cadmium was recorded in the kidneys, especially in the post-exposure period, which can point to an intensive elimination from the body. The prolonged exposure to cadmium compounds also led to the increase of copper levels and decrease in iron and selenium levels in the kidneys. The most significant changes were recorded after exposure to CdS NP, especially of 4–6 nm size, as compared to CdCl2. The intensive accumulation of cadmium and imbalance of trace elements can play a leading role in hepatotoxic and nephrotoxic effects of cadmium compounds in nano- and ionic form.

Conclusions. Determination of the content of cadmium, macro- and microelements in the inner organs allows assessing cadmium toxicokinetics in the body, predicting the pathogenetic features of its toxic effects, especially in the post-exposure period, as well as studying the peculiarities of the pathogenesis of its organotoxic action.

Key words: Cadmium, Nanoparticles, Liver, Kidneys, Zinc, Selenium, Copper, Iron, Calcium

References

  1. Liu, L., Sun, M., Li, Q. et al. 2014, "Genotoxicity and Cytotoxicity of Cadmium Sulfide Nanomaterials to Mice: Comparison Between Nanorods and Nanodots", Environmental Engineering Science, v. 31 (7), pp. 373– 380.
  2. Kozhevnikova, N.,S., Vorokh, A.,S., Uritskaya, A.,A.2015, "Cadmium sulfide nanoparticles prepared by chemical bath deposition", Russian Chemical Reviews, v. 84 (3), pp. 225–250 (in Russian).
  3. Jarup, L., Berglund, M., Elinder, C. et al. 1998, "Health effects of cadmium exposure – a review of the literature and a risk estimate", Scandinavian Journal of Work, Environment & Health, v. 1, pp. 1–52.
  4. Godt, J., Scheidig, F., Grosse-Siestrup, C. et al. 2006, "The toxicity of cadmium and resulting hazards for human health", Journal of Occupational Medicine and Toxicology, v. 1, рp. 22–28, doi: 10.1186/1745-6673-1-22.
  5. Åkesson, A., Barregard, L., Bergdahl, I. A. et al. 2014, "Non-renal effects and the risk assessment of environmental cadmium exposure", Environmental Health Perspectives, v. 122 (5), р. 431–438.
  6. Jie, Liu, Wei, Qu, Kadiiska, M. B. 2009, "Role of oxidative stress in cadmium toxicity and carcinogenesis", Toxicology and Applied Pharmacology, v. 238 (3), pp. 209–214.
  7. Huff, J., Lunn, R. M., Waalkes, M. P. et al. 2007, "Cadmium-induced Cancers in Animals and in Humans", International Journal of Occupational and Environmental Health, v. 13 (2), pp. 202–212.
  8. Adams, S. V., Passarelli, M. N., Newcomb, P. A. 2012, "Cadmium exposure and cancer mortality in the Third National Health and Nutrition Examination Survey cohort", Occupational and Environmental Medicine, v. 69 (2), рр. 153–156.
  9. Prozialeck, W. C., Edwards, J. R. 2010, "Early biomarkers of cadmium exposure and nephrotoxicity", Biometals, v. 23 (5), pp. 793-809. doi: 10.1007/s10534010-9288-2.
  10. He, L., Wang, B., Hay, E. B., Nebert. D. W. 2009, "Discovery of ZIP transporters that participate in cadmium damage to testis and kidney", Toxicology and Applied Pharmacology, v. 238, pp. 250–257.
  11. Prozialeck, W. C., Edwards, J. R. 2012, "Mechanisms of Cadmium-Induced Proximal Tubule Injury: New Insights with Implications for Biomonitoring and Therapeutic Interventions", Journal of Pharmacology and Experimental Therapeutics, v. 343 (1), pp. 2–12. doi: 10.1124/jpet.110.166769.