Kuzmina L. P., Izmerova N. I., Kolyaskina M. M., Lazarashvili N. A., Petinati Y. A.
Federal State Budgetary Institution Research Institute of Occupational Health of the Russian Academy of Medical Sciences, Moscow, Russian Federation
Purpose of work. To examine the role of polymorphic variants of genes of the system of xenobiotic biotransformation in mechanisms of formation and development of occupational allergic dermatoses.
Methods of research. The study included 201 workers, contacted with sensitizing and iritative substances with the established ooccupational allergic dermatoses. Gene polymorphisms of cytochrome P-450: cytochrome P-450 1A1 (CYP1A1 (* 2C I462V (A> G)), and cytochrome P-450 3A4 (CYP3A4 (*1B A> G), glutathione-S-transferase M1 (GSTM1 gene), glutathi- one-S-transferase T1 (GSTT1 gene), microsomal epoxidehydrolase 1 (ERNH1 of polymorphisms H139R (A-415G) and Y113H (T-337C) were used in order to assess mechanisms of formation and development of occupational allergic dermatoses by the method of polymerase chain reaction (PCR) and pyrosequencing reaction.
Results. The study of polymorphic genes of xenobiotic biotransformation system in patients with occupational allergic dermatoses showed significantly higher percentage of polymorphic variants of genes CYP 1A1*2C and EPHX1 A-415G as compared with the population control. The combination of 3 or more adverse hetero-and homozygous alleles CYP 1A1, CYP3A4, ERNH1 and deletions of genes GSTM1 and GSTT1 are characterized by earlier development (after working in hazardous conditions up to 5 years) of severe courses and unfavorable prognosis for occupational skin diseases.
Conclusions. The obtained results can make the basis for development of personalized treatment and preventive measures with the use of antioxidant therapy in individuals, working in allergo-hazard productions and in patients with occupational allergic dermatoses.
Key words: genes of biotransformation, occupational allergodermatosis, risk assessment