https://doi.org/10.33573/ujoh2018.01.061

Andrushchenko T. A.1, Goncharov S. V.2, Dolinchuk L. V.3, Dosenko V. E.2

POLYMORPHISM OF XPD GENE (RS799793) AND BRONCHOPULMONARY PATHOLOGY IN WORKERSOF HARMFUL AND DANGEROUS BRANCHES OF INDUSTRY IN UKRAINE

1State institution "Kundiiev institute of occupational health of the National academy of medical sciences of Ukraine", Kyiv

2Bogomoletz institute of physiology of the NAS of Ukraine, Kyiv

3Bogomolets National Medical University, Kyiv

Full article (PDF), UKR

Introduction. The most common among occupational diseases in Ukraine are diseases of the bronchopulmonary system. In the structure of harmful and dangerous work­related and occupational factors there are available diseases, which can cause disorders in the system of DNA repair and inducing mutagenesis.

Purpose of the study was to investigate the distribution of frequencies of alleles and genotypes of the repair gene XPD (rs799793) in patients with pathology of the bronchopulmonary system in workers of dusty professions at cement enterprises and coal mines of Ukraine.

Materials and methods. The study covered two categories of workers: workers of the cement­concrete enterprises of Ukraine (n = 92) and coal miners (n = 98). A real­time polymerase chain reaction method was used to determine genotypes of the XPD gene (rs799793). The results were statistically processed using programs of Orion 7.0, Statistica, and Excel 2000. Results. The frequencies of genotypes of XPD gene (rs799793) were established in occupational groups of miners and workers of the cement industry of Ukraine. The OR value of the minor allele XPD*Asn in workers of the cement enterprises indicated the correlation of disorders in the excitatory repair system of nucleotides with the risk of bronchopulmonary pathology development (OR = 1.34, 95 % CI: 0.69–2.59), whereas the dominant XPD*Asp allele promoted a protective actiont for its carriers (OR = 0.75, 95 % CI: 0.39–1.44). When studying the frequencies of genotypes of the XPD gene (rs799793) it is established that the XPD*Asn/Asn genotype is associated with a risk of the bronchopulmonary pathology and the inhibition of the efficiency of the excision repair of nucleotides in the population of workers at Ukrainian cement plants (OR = 2.15, 95% CI: 1.71–2.70, p ≤ 0.01, c2 = 6.35). Conclusions. As a result of the molecular genetic study of polymorphism of the XPD gene (rs799793) it was established that the XPD*Asn/Asn genotype was associated with the risk of developing the bronchopulmonary pathology and suppressing the efficiency of the excision repair of nucleotides in the population of workers at cement­concrete enterprises of Ukraine. Among the employees of the asbestocement enterprises the carriers of allele XPD*Asn promoted the increased risk of development of the bronchopulmonary pathology, and the carriage of allele XPD*Asp caused a protective actiont on development of the above pathology.

Key words: bronchopulmonary pathology, molecular-genetic markers

References

  1. Кuzmina L. P., Izmerov N. F., Dinisov E. N. (2003), "Biochemical and genetic indicators of individual sensitivity to occupational hazards: Occupational health risk for workers", Guide, Trovant, Moscow, Russia, 329–334.
  2. Кundiiev Y. I. and Nahorna A. (2005), "Occupational morbidity in Ukraine in the dynamics of a long­term examination", Ukr. J. Occup. Health, 1, 3–11.
  3. Urzhumov P. V., Pogodina A. V. and Akleev A. V. (2013), "Polymorphisms of NBS1 and PARP1 genes and DNA repair efficiency", Vestnik Chelyabinskogo gosudarstv ennogo universiteta, 298 (7), Biologiya, 2, 107–108.
  4. Kiffmeyer W. R., Langer E. and Davies S. M. (2004), "Genetic Polymorphisms in the Human Population", Cancer, 100, 2, 411–417. https://doi.org/10.1002/cncr.11913
  5. Kuschel B., Auranen A., McBride S., Novik K. L. and Antoniou A. (2002), "Variants in double­strand break repair genes and breast cancer susceptibility", Hum Mol Genet, 11, 1399–440. https://doi.org/10.1093/hmg/11.12.1399
  6. Pavanello S. and Clonfero E. (2004), "Individual susceptibility to occupational carcinogens: the evidence from biomonitoring and molecular epidemiology studies", G Ital Med Lav Ergon, 26, Oct­Dec., 4, 311–21.
  7. Shin A., Lee K. M., Ahn B., Park C. G., and Noh S. K. (2008), "Genotype­phenotype relationship between DNA repair gene genetic polymorphisms and DNA repair capacity", Asian Pac J Cancer Prev, 9, 501–505.

ORCID ID of co-authors and their contribution to the preparation and writing of the article:

Andrushchenko T.A. (ORCID ID 0000000328588267) - collection of primary material, participation in research implementation, statistical processing of results;

Goncharov SV - participation in the research implementation;

Dolinchuk L.V. - analysis of contemporary world and domestic literature;

Dosenko V. E. - the choice of methods and research program.