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STUDY OF METALS NANOPARTICLES CYToToXiC iMPACT ON THE HUMAN CELLS LINES IN IN VITRO EXPERIMETS

1(25) 2011

DOI xxxxxxxx

Marchenko M. L.1, Bezdenezcnih N. A.2, Fahmi M.3

STUDY OF METALS NANOPARTICLES CYToToXiC iMPACT ON THE HUMAN CELLS LINES IN IN VITRO EXPERIMETS

1 Institute for Occupational Health of AMS of Ukraine, Kiev
2 Institute of experimental pathology, oncology and radiobiology n. R.E. Kaveckiy of NAN of Ukraine, Kiev
3 National Bogomolets Medical University, Kiev

Full article (PDF), UKR

With the rapid development of nanotechnology are not only significant achievements in the field of medicine, various industries and precise technology, but also undesirable effects on human health and the environment. In this regard, there is an important problem in the development of state sanitary-hygienic norms and standards that will regulate concentration of nanoparticles in the working area and environment. Since the toxicity of nanoparticles depends not only on the chemical composition, but also on their size, surface characteristics, electro-magnetical parameters and inner structure the stages of toxicological and hygienic evaluation of nanomaterials is needed differentiated approach, which in turn requires a significantly larger number of animals in toxicological studies than in the evaluation of substances micro sizes of the same nature. Thus, the toxicity studies on nanoparticles in vitro cultures of human cells may not only help reduce the number of animals in research in vivo, but also provide valuable information about possible risks to a living organism and the environment in nanoparticles exposure conditions. In this regard, we conducted pilot studies of several metals nanoparticles acute toxic effects in cultures of human cells — A-549, HaCaT, Colo-205, U-373. Our results respond to the scientific literature facts that demonstrate the spectacular damaging effects of nanoparticles on lung tissue and human skin as well as animals in vivo.

Key words: nanoparticles, cultures of human cells, cytotoxic impact, ЕС50

References

    1. Borisenko V. Nanotekhnologii: etapy razvitiya / V. Borisenko, N. Tolochko // Nauka i innovatsii.- 2008.- T 12, No 70.- S. 53-58.
    2. Cavalcanti A. Medical Nanorobot Architecture Based on Nanobioelectronics / [Cavalcanti A., Shirinzadeh B., Freitas R.A. Jr., Kretly L.C.] // Recent Patents on Nanotechnology- 2007.- V. 1 (1) - P. 1-10.
    3. Elder A. Tiny Inhaled Particles Take Easy Route from Nose to Brain / Elder A. // Report of University Rochester medical center.- 2006.- 1 p.
    4. Toxicity and penetration of TiO2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposure / Wu J., Liu W., Xue C. [et al.] // Toxicology letters.- 2009.- V. 191, No 1.- P. 1-8.
    5. Paull J. Nanotechnology: The Next Challenge for Organics / J. Paull, K. Lyons // Journal of Organic Systems.- 2008.- No 3.- Р 22-28.
    6. Shetty R. C. Potential pitfalls of nanotechnology in its applications to medicine: immune incompatibility of nanodevices / R. C. Shetty // Med. Hypotheses.- 2005.- V. 65, No 5 - P. 998-999.
    7. Smith Rebecca. Nanoparticles used in paint could kill, research suggests / Smith Rebecca.- London: Telegraph, 2010.- P. 4.
    8. Bowman D. Nanotechnology: Mapping the Wild Regulatory Frontier / D. Bowman, G. Hodge // Futures.- 2006.- No 38.- Р. 1060-1073.
    9. Nagender R. P. Silver or silver nanoparticles: a hazardous threat to the environment and human health? / Reddy Panyala Nagender, Eladia Maria Pena-Mendes, Hosef Havel // Journal of applied biomedicine.- 2008.- No 6.- Р 117-129.
    10. Copper development association. - United Kingdoms: 1992.-Access :http://wwwcopperinfo.co.uk/health/downloads/pub- 96-copper-in-human-health.pdf,
    11. Acute toxicological effects of copper nanoparticles in vivo / Zhen Chen [et al.] // Elsevier - Toxicological letters.- 2006.- No 163.- P. 109-120.
    12. Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro / E. Meghan [et al.] // Environmental Health Perspectives.- 2010.- No 118.- Р. 407-413.
    13. El-Ansary A. On the toxicity of therapeutically used nanoparticles: an overview / S. El-Ansary, Al-Daihan // Hindawi Publ. Corp.- Journal oftoxicology.- 2009.- No 10.- Р. 9.
    14. Rani A. Anti-proliferative activity of silver nanoparticles / Asha Rani, M. Prakash Hande, Suresh Valiyaveettil // BMC Cell Biology.- 2009.- No 10.- P. 65.
    15. Risk Assessment Information System, sponsored by the U.S. Department of Energy (DOE), Office of Environmental Management, Oak Ridge Operations (ORO) - Office The University of Tennessee, 2009.- : http://rais.ornl.gov/ tox/profiles/molybdenum_c_V1.html,
    16. Wennig, R. Molybdenum. In: Handbook on Toxicity of Inorganic Compounds / R. Wennig, N. Kirsch - H.G. Seiler and H. Sigel, eds. Marcel Deker, Inc. New York, - 1988.- P. 437-447.