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1(25) 2011

Marchenko M. L.1, Bezdenezcnih N. A.2, Fahmi M.3


1 Institute for Occupational Health of AMS of Ukraine, Kiev
2 Institute of experimental pathology, oncology and radiobiology n. R.E. Kaveckiy of NAN of Ukraine, Kiev
3 National Bogomolets Medical University, Kiev

Full article (PDF), UKR

With the rapid development of nanotechnology are not only significant achievements in the field of medicine, various industries and precise technology, but also undesirable effects on human health and the environment. In this regard, there is an important problem in the development of state sanitary-hygienic norms and standards that will regulate concentration of nanoparticles in the working area and environment. Since the toxicity of nanoparticles depends not only on the chemical composition, but also on their size, surface characteristics, electro-magnetical parameters and inner structure the stages of toxicological and hygienic evaluation of nanomaterials is needed differentiated approach, which in turn requires a significantly larger number of animals in toxicological studies than in the evaluation of substances micro sizes of the same nature. Thus, the toxicity studies on nanoparticles in vitro cultures of human cells may not only help reduce the number of animals in research in vivo, but also provide valuable information about possible risks to a living organism and the environment in nanoparticles exposure conditions. In this regard, we conducted pilot studies of several metals nanoparticles acute toxic effects in cultures of human cells — A-549, HaCaT, Colo-205, U-373. Our results respond to the scientific literature facts that demonstrate the spectacular damaging effects of nanoparticles on lung tissue and human skin as well as animals in vivo.

Keywords: nanoparticles, cultures of human cells, cytotoxic impact, ЕС50


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