Rudenka K. L.
STUDY OF TROFIN ACTION IN ACUTE AMINOGLYCOSIDE OTOTOXICITY (A MORPHOLOGICAL RESEARCH)
State Institution "O. S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine", Kiev
Full article (PDF), UKR
Introduction. The impact of noise and vibration on the human body is one of the major hazards/noxious agents of the environmental and industrial production, causing a sensorineural hearing loss (SNHL). According to the WHO data of 2002, noise is the second largest occupational risk factor by health and social damage.
Objective. To determine the effectiveness of trofin action in experimental sensorineural hearing loss, induced by aminoglycoside antibiotics.
Materials and methods. Experimental studies were performed on 37 mature guinea pigs. A modeling of SNHL was made using gentamicin, which was administered daily for 14 days in the dose of 50 mg/kg (group 2). At the background of gentamicin administration, animals of 3–6 groups received trofin at the dose of 0,4 ml/kg intramuscularly and intraperitoneally.
Results. Significant disorders in the microcirculatory elements of the bone and the membranous labyrinth have been observed on the background of a 14-fold administration of gentamicin. Thus, the blood vessels demonstrated congestion; their lumens were blood-clotted. The spiral ligament was very swollen and of uneven thickness; sludge was observed in capillaries. Hair cells and cochlear were so swollen, that their line was broken. The injection of trofin completely neutralized a negative effect of aminoglycoside antibiotic, irrespective of the method of administration.
Conclusions. The results of experimental studies give occasion to the hope for the possibility of effective use of trofin for treatment of patients with sensorineural hearing loss.
Key words: hearing loss, aminoglycoside antibiotic, trofin, guinea pigs, inner ear
- Baranenko, B. O., Tsymbalyuk, V. I., Vasilyeva, I. G., Chopyk, N. 2012, "Effect of transplantation of fetal tissues on serotoninergic systems in the brain of experimental animals as a result of a brain injury", Ukrainian neurosurgical journal, no. 2, pp. 18–23 (in Ukrainian). https://doi.org/10.25305/unj.59043
- Belousova, A. O., Timen, G. E., Tsymbalyuk, V. I. et al. 2013, "Impact trofin and glutargin on morphological state of the inner ear in guinea pigs in experimentally induced sensorineural hearing loss", Zhurn. vushnykh, nosovykh i horlovykh khvorob, no. 3, pp. 19–20 (in Ukrainian).
- Denisov, E. I., Ilkayeva, E. N., Kuryerov, Y. Y. 2005, "Principles and criteria of the standard on occupational health on prevention of hearing loss", Meditsina truda i promyshlennaya ekologiya, no. 2, pp. 16 (in Russian).
- Kundiyev, Y. I,. Nahorna, A. M., Chernyuk, V. I. 2005, "Strategy of providing safe work conditions and promotion of the population health in Ukraine for 2006– 2010", Ukr J Occup Health, no. 3–4, pp. 4–10 (in Ukrainian).
- Magomedov, M. 1998, "The use of fetal tissue transplantation in otolaryngology. Analysis of the problems and prospects of development", Vestnik otorinolaringologii, no. 2, pp. 16–23 (in Russian).
- Magomedov, M. 1997, The use of fetal tissues in treatment of chronic sensorineural hearing loss, Proceedings of the International Scientific-Practical Conference on the 75th anniversary of the Chair of Otolaryngology and Clinics of the Dnipropetrovsk Medical academy. Dnipropetrovs'k, pp. 140–141 (in Russian).
- Mashkovsky, M. D. Drugs, (15th ed., rev., corr. and add.). Moscow: RIA "Novaya volna", 1206 p. (in Russian).
- Semenova, V. M., Tsimbalyuk, V. I., Pichkur, L. D. 2008, "Comparative cytostructural assessment of the growth of human embryonic neural tissue in conditions of cultivation by various ways of storage", Ukrainian neurosurgical journal, no. 4, pp. 68–71 (in Ukrainian). https://doi.org/10.25305/unj.108393
- Sipityj, V. I., Kochin, O. V., Petrenko, O. 2005, "Experimental morphological and biochemical grounding of the use of cryopreserved embryonic nerve cells in the surgical treatment of epilepsy", Ukrainian neurosurgical journal, no. 1, pp. 52–56 (in Ukrainian).
- Timen, G. E., Tsymbalyuk, V. I., Byelousova, A. O. et al.2012, "Study of the effect of glutargin and trofin the state of the inner ear in guinea pigs after experimentally induced aminoglycoside ototoxicity", Zhurnal vush., nosovykh I gorlovykh khvorob, no. 5, pp. 137–138 (in Ukrainian).
- Tsymbalyuk, V. I., Vasiliev, I. G., Oleksenko, N. 2001, "Effect of extract of fetal nerve tissue on the contents of gamma-aminobutyric acid in the culture of nerve cells", Transplantologia,v. 2, no.1, pp. 70–73 (in Ukrainian).
- Tsymbalyuk, V. I., Lisyanyi, M. I., Markova, E. V., Pichkur, L. D. 2003, "Results of surgical treatment of experimental allergic encephalomyelitis", Transplantologia, v. 4, no.1, pp. 115–117 (in Ukrainian).
- Tsymbalyuk, V. I., Latyshev, D. Y., Pichkur, L. D. 2005, "Impact of neurotransplantation on the course of seizures in patients with apallic syndrome and its consequences", Ukrainian neurosurgical journal, no. 4, pp. 90–93 (in Ukrainian).
- Tsymbalyuk, V. I., Vasilieva, I. G., Chopik, N. G. et al. 2003, "Expression of neurotrophic factors in the embryonic human brain of 5-9 weeks of gestation", Ukrainian neurosurgical journal, no. 3, pp. 13–16 (in Russian).
- Tsymbalyuk, V. I., Nosov, T., Chebotarev, L. L., Vasyuta, V. A. 2004, "Electrophysiological and morphological indicators of the state of the visual analyzer in the dynamic of Trofin use in methanol intoxication", Ukrainian neurosurgical journal, no. 3, pp. 97–102 (in Russian).
- Huisman, M. A., Rivolta, M. N. 2012, "Neural crest stem cells and their potential application in a therapy for deafness", Frontiers Bioscience (School Ed), v. 4, pp. 121–132. https://doi.org/10.2741/s255
- Kersigo, J., Fritzsch, B. 2015, "Inner ear hair cells deteriorate in mice engineered to have no or diminished innervations", Frontiers in aging neuroscience, v. 7, pp. 570–587. https://doi.org/10.3389/fnagi.2015.00033
- Rivolta, M. N. 2013, "New strategies for the restoration of hearing loss: challenges and opportunitie", British Medical Bulletin, v. 105, pp. 69–84. https://doi.org/10.1093/bmb/lds035