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Features of using biological insecticidal preparations based on Bacillus thuringiensis, Metarhizium anisopliae (robertsi), Beauveria bassiana in agricultur

ISSN 2223-6775 Ukrainian journal of occupational health Vol.20, No 1, 2024


https://doi.org/10.33573/ujoh2024.01.044

Features of using biological insecticidal preparations based on Bacillus thuringiensis, Metarhizium anisopliae (robertsi), Beauveria bassiana in agricultur

Prygunova V.V., Dontsova D.O., Kovalenko V.F., Ishcheykin K.Ye.
State Institution «Kundiev Institute of Occupational Medicine of the National Academy of Medical Sciences of Ukraine», Kyiv


Full article (PDF), UKR

Introduction. The population of planet Earth largely depends on agricultural production of plants and animals to meet the food demand. However, humans are not the only organisms that eagerly consume these products. Every year, insect pests cause serious damage to agriculture. Loss of productivity and the high cost of chemical pesticides increase the cost of production. Continuous use of chemical pesticides creates selective pressure, leading to the development of pest resistance. Excessive residues of chemical pesticides also pollute the environment, including soil and water. Therefore, biological control of insect pests in agriculture becomes more important due to food safety and environmental concerns. In this regard, bacterial insecticides offer a better alternative to chemical pesticides. This not only helps establish food security by combating insect pests but also ensures the safety of food products.

The aim of the study is to substantiate the conditions for the safe application of biological insecticidal preparations in agriculture.

Materials and methods of the study. Objects of study: Biological insecticidal preparation INSECTA BBM, LLC (active ingredients – Bacillus thuringiensis, not less than 1,0×109 CFU/g, Metarhizium anisopliae (robertsi), not less than 1,0×108 CFU/g, Beauveria bassiana, not less than 1,0×108 CFU/g), toxicological and hygienic assessment from the perspective of safety for human health and the natural environment, expert-analytical methods for studying scientific information on toxicological properties., hygiene, risk assessment

Results. Entomopathogenic fungi of the Metarhizium anisopliae and Beauveria bassiana preparations are capable of penetrating through the chitinous and other coverings of pests, affecting the fatty tissue and gastrointestinal tract. They decompose chitin and form a cottony mycelial growth on the bodies of pests, causing disruption of all organism functions. The chitinases of fungi decompose the chitin of phytopathogens, contributing to the destruction of plant disease agents. Fungi have the property of establishing themselves in the soil, providing the biological preparation with long-lasting action – suppressing the reproduction of insects. A promising direction of research is the combination of strains for the production of preparations that would be universal and affect more species of insect pests, as well as prolong the shelf life of entomopathogenic preparations.

Conclusions. The use of mycoinsecticides represents a crucial alternative for combating a complex of soil pests and can reduce the use of broad-spectrum pesticides on vegetable crops, thereby enhancing natural control by increasing the number of natural enemies. Scientific data regarding the features of technology for producing biological insecticidal preparations based on Bacillus thuringiensis, Metarhizium anisopliae (robertsi) and Beauveria bassiana have been summarized. Based on the results of the toxicological evaluation of the drug, it can be concluded that the biological product is safe enough for human health and the environment.

Keywords: toxicological and hygienic assessment, biological preparation with insecticidal action, Bacillus thuringiensis, Metarhizium anisopliae (robbertsi), Beauveria bassiana, agriculture.

References

  1. Bonaterra A, Badosa E, Daranas N, Francés J, Roselló G, Montesinos E. Bacteria as Biological Control Agents of Plant Diseases. Microorganisms. 2022;10(9):1759. DOI: https://doi.org/10.3390/microorganisms10091759.
  2. Volkohon VV, Nadkernychna OV, Kovalevska TM, Tokmakova LM, et al. [Microbial preparations in agriculture. Theory and practice: Monograph]. Volkohon VV, editor. Kyiv: Agrarian Science; 2006. 312 p. Ukrainian.
  3. Bilay VI. [Microscopic fungi – producers of antibiotics]. Kyiv: Academy of Sciences of the Ukrainian; 1961. 181 p. Russian.
  4. Gupta S, Dikshit AK. Biopesticides: An ecofriendly approach for pest control. Journal of Biopesticides. 2010;3(1):186-8.
  5. Usta C. Microorganisms in Biological Pest Control – A Review (Bacterial Toxin Application and Effect of Environmental Factors). In: Silva-Opps M, editor. Current Progress in Biological Research. IntechOpen; 2013. DOI: https://doi.org/10.5772/55786. Available from: https://www.intechopen.com/chapters/44118.
  6. Passport of the strain of microorganism / Dossier of the company LLC «IMEX AGRO», Ukraine.
  7. US Environmental Protection Agency. Office of Pesticide Programs. Biopesticides registration action document: Metarhizium anisopliae strain F52 (PC Code 029056). 2000. 40 p.
  8. Faria MR, Wraight SP. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. Biol. Control. 2007;43(3):237-56. DOI: https://doi.org/10.1016/j.biocontrol.2007.08.001.
  9. Rajula J, Karthi S, Mumba S, Pittarate S, Thungrabeab M, Krutmuang P. Current status and future prospects of entomopathogenic fungi: A potential source of biopesticides. In: Surajit De Mandal, Ajit Kumar Passari, editors. Recent Advancement in Microbial Biotechnology: Agricultural and Industrial Approach. Academic Press; 2021. P. 71-98. DOI: https://doi.org/10.1016/B978-0-12-822098-6.00013-6.
  10. Chintkuntlawar PS, Pramanik A, Solanki R, Rathod Metarhizium anisopliae: New Trend Entomopathogenic Fungus for Management of Sucking Pests in Vegetable Crops. Popular Kheti. 2015;3(1):98-101.
  11. Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine[Conclusion on the study of the virulence of Beauveria bassiana L-8 strain in the model of white mice]. 2021. 4 p. Ukrainian.
  12. Database/Pesticides/dictionary/pesticide. [Internet]. Available from:http://www.pesticidy.ru/ dictionary/pesticide.
  13. Imoulan A. Hussain M, Kirk PM, El Meziane A, Yao Yi-J. Entomopathogenic fungus Beauveria: Host specificity, ecology and significance of morpho-molecular characterization in accurate taxonomic classification. Journal of Asia-Pacific Entomology. 2017; 20(4): 1204- DOI: https://doi.org/10.1016/J.ASPEN.2017.08.015.
  14. Kuznetsova LN. [Domestic entomopathogenic biological products based on Bacillus thuringiensis]. Bulletin of the Institute of Agricultural Microbiology. 1999;4:22-4. Ukrainian.
  15. Kandybin NV. [Biopesticides. Theory and practice]. Zashchyta rastenyi.1991;1:10-3. Russian.
  16. Omelyanets TG, Golovach TM. [The estimation of pathogenic action of biopreparation on the basis of Bacillus thuringiensis on warm-blooded organism]. Environment & Health. 2008;2:65-8. Ukrainian.
  17. Medved LI, editor. [Reference book on pesticides (hygiene of application and toxicology)]. Kyiv: Urozhay; 1974. 448 p. Russian.
  18. IAS «Agrarians together». Pesticides and agrochemicals base. Active factor: bacillus-thuringiensis-var-kurstaki. [Internet]. [cited 2024 Mar 01]. Available from: https://agrarii-razom.com.ua/active-ingredients/bacillus-thuringiensis-var-kurstaki
  19. Database/Pesticides/Active factor: metarhizium_anisopliae. [Internet]. Available from:https://www.pesticidy.ru/active_substance/metarhizium_anisopliae.
  20. Database/Pesticides/Active factor:Beauveria_bassiana. [Internet]. Available from:https://www.pesticidy.ru/active_substance/Beauveria_bassiana.
  21. McGuire AV, Northfield TD. Tropical Occurrence and Agricultural Importance of Beauveria bassiana and Metarhizium anisopliae: mini review article. Front. Sustain. Food Syst. 2020, Jan29;4(6). DOI: https://doi.org/10.3389/fsufs.2020.00006.
  22. State Sanitary Rules and Norms DSanPiN 8.8.1.2.002-98. [Hygienic Classification of Pesticides by Degree of Hazard]. In: [Organization and Implementation of State Sanitary and Epidemiological Supervision over the Use of Pesticides and Agrochemicals]: Collection of Basic Legislative and Regulatory Documents. 1999. p. 249-67. Ukrainian.