https://doi.org/10.33573/ujoh2015.01.010

Dolinchuk L.V., Basanets A.V., Andrushchenko T. A.

ROLE OF MMP-2 GENE POLYMORPHISM IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE IN MINERS

SI «Institute for Occupational Health of NAMS of Ukraine», Kyiv

Full article (PDF), UKR

Introduction. In recent years an increased incidence of chronic obstructive pulmonary disease (COPD) is observed in workers of coal, metallurgical and mining industries. Nowadays, a priority task is the search for more effective methods of prevention, which would be enable to prevent the development of the disease. With this in view, the determination of susceptibility of genetic markers to COPD allows to open new possibilities for prevention of this disease in risk groups.

Objective of research. To define a role of MMP-2 gene polymorphism (C-1306T) in the development of chronic obstructive pulmonary disease (COPD) in order to improve early detection and primary prevention of the disease.

Materials and methods. A real-time polymerase chain reaction (RT-PCR) was performed to detect a polymorphism C-1306T of the matrix metalloproteinase-2 (MMP-2) gene. The study covered 72 miners with COPD, and 79 miners without the respiratory system pathology.

Results. The frequency of prevalence of MMP-2 gene polymorphism C-1306T is similar to Caucasian population. This study made it possible to establish the association between the major C-allele (OR = 0,58; 95 % CI: 0,33–1,04; p < 0,05; c2 = 3,78) and СС genotype (OR = 0,55; 95 % CI: 0,27–1,12; p = 0,07) of MMP-2 gene with the resistance to development of COPD in miners. The presence in the genome minor T allele (OR = 1,71; 95 % CI: 0,96–3,05; p < 0,05; c2 = 3,78) allows to determine a predisposition to risk of COPD in miners.

Conclusion. The results of the research make it possible to open new perspectives for improving measures of primary prevention of COPD, based on determining the genetic predisposition to COPD development.

Key words: chronic obstructive pulmonary disease, genetic markers, MMP-2, miners

References

  1. Kundiiev, Y. I., Nagornaya, A. M., Sokolova, M. P., Kononova, I. G. 2013, «Dynamics of occupational morbidity in Ukraine and experience of the Institute for Occupational Health of NAMS of Ukraine», Ukrainian Journal of Occupational Health, no. 4 (37), pp. 11–22 (in Ukrainian).
  2. Graber, J. M. et. al. 2012, «Results from a Ukrainian-US collaborative study: prevalence and predictors of respiratory symptoms among Ukrainian coal miners», Am J. Ind Med., no. 55 (12), pp. 1099–109. https://doi.org/10.1002/ajim.21997
  3. Nair, K., Ghushchyan, V., Van Den Bos, J. et. al. 2012, «Burden of illness for an employed population with chronic obstructive pulmonary disease», Popul Health Manag., no.15 (5), pp. 267–75. https://doi.org/10.1089/pop.2011.0049
  4. Global Initiative for Chronic Obstructive Lung Disease. Global Strategy or the Diagnosis, Management and Prevention of Chronic Obstructive Lung Disease. HLBI/ WHO workshop report. Last update 2011. Available in: http://www.goldcopd.com.
  5. Price, S. J., Greaves, D. R., Watkins, H. 2001, «Identification of novel, functional genetic variants in the human matrix metalloproteinase-2 gene: role of Sp1 in allele-specific transcriptional regulation», J. Biol. Chem., no. 276. (10), pp. 7549–7558. https://doi.org/10.1074/jbc.M010242200
  6. National Center for Biotechnology Information Available in: http:http://www.ncbi.nlm.nih.gov/ projects/SNP/.
  7. Ohnishi, K., Takagi, M., Kurokawa, Y., et al. 1998, «Matrix metalloproteinase-mediated extracellular matrix protein degradation in human pulmonary emphysema», Lab. Invest., no. 78, pp. 1077–1087.
  8. Seagrave, J., Barr, E. B., March, T. H. 2004, «Effects of cigarette smoke exposure and cessation of inflammatory cells and matrix metalloproteinase activity in mice», Exp. Lung Res., no. 30, pp. 1–15. https://doi.org/10.1080/01902140490252858